Depth Filters
The Alioth Alidep® Depth Filter is designed primarily for clarification steps in pharmaceutical processes, offering enhanced flexibility for large-scale production through its disposable format.
The Alidep® Depth Filter is available in a range of media combinations, typically composed of cellulose, filter aids, and positively charged resins. It delivers a high dirt-holding capacity, making it well-suited for clarifying cell culture fluid. The filter progressively retains intact cells and cell fragments, reduces turbidity, and effectively adsorbs impurities such as DNA, host cell proteins (HCP), and endotoxins—thereby reducing the burden on downstream purification steps.
With multiple product specifications available, the Alidep® Depth Filter supports applications from laboratory and pilot-scale through to manufacturing-scale.
Key Features and Benefits
Disposable design eliminates the need for cleaning validation
Modular design adapts to current mainstream clarification holders and systems
Small hold-up volume facilitates easy product recovery, improving yield
Flexible media selection optimize customer product development
Easy operation, small footprint, and space-saving design
Typical Applications
Clarification of CHO cell culture
Clarification in vaccine production process
Clarification of yeast fermentation broth (after centrifugation)
Clarification in plasmid production process (E. coli lysate)
Clarification of lentivirus and adeno-associated virus culture fluid
Product Specifications
Product Specifications
| Product Specifications | Product Series | Alidep® process development scale (L series) | Alidep® laboratory scale (P series) | Alidep® production scale (M series) |
| Effective Filtration Area | 23 cm² | 270 cm²、540 cm² | 0.11 m²、0.55 m²、1.1 m² | |
| Overall Dimension | 9.4×7.1×4.3 cm | 22×14×(7.4~9.6) cm | 62×32×(3.1~12.4) cm | |
| Materials of Construction | Inlet, Outlet and Exhaust Port | Luer connector: Polypropylene | 6 mm hose connector: Glass fiber-filled polypropylene | Flat end connector: Glass fiber-filled polypropylene |
| Frame and Cover Material | Glass fiber-filled polypropylene | |||
| Filter Plate | Cellulose, Filter Aid, Resin or Cellulose | |||
| Supporting Layer | Polypropylene | |||
| O-ring | Silicone | |||
| Working Characteristics | Aerosol Challenge Test | Aerosol leakage < 0.01% | ||
| Maximum Operating Pressure | 3.5 bar @ 40°C | |||
| Maximum Forward Differential Pressure | 3.0 bar @ 25℃ | |||
| Maximum Reverse Differential Pressure | 2.0 bar @ 25℃ | |||
| Sterilization Resistance | Autoclave: 125℃ 60 min，2cycles | |||
| Indirect Food Additive | All component materials meet the requirements of Indirect Food Additive cited in FDA 21 CFR 177-182 | |||
| Toxicity | The raw materials of all Alidep® Depth Filter components, including filter plates ( mainly made from cellulose, filter aids and resin ), frames and covers made from glass fiber-filled polypropylene, depth supporting nets made from polypropylene, and silicone O-rings, meet the biological safety requirements refering to USP <88> Class VI plastic | |||
| Bacterial Endotoxin | The endotoxin level of rinsing liquid is < 0.25 EU/mL, which complies with the requirements for water for injection in Chinese Pharmacopoeia and United States Pharmacopoeia | |||
Pore Size Range
Pore Size Range
| Name of medium | Application | Features | Composition |
| Double-layer HD Series | Primary clarification of cell culture fluid | Two-stage filter plate and open pore, which can retain large particles and fragments | Cellulose, filter aids and resin |
| Double-layer HA Series | Secondary clarification of cell culture fluid | Double-stage filter plate and dense structure, which can achieve more strict turbidity control | Cellulose, filter aids and resin |
| Double-layer HC Series | Direct clarification of cell culture fluid | Double-stage filter plate, which is designed to clarify material liquid with a low cell density | Cellulose, filter aids and resin |
| Double-layer CE Series | Clarification of plasmids and virus particles, etc. | Double-stage filter plate, which features low adsorption | Cellulose |
Pore Size Range
Test Results
Conductivity Test Results
pH Test Results
TOC Test Results
Test Results of Flow Differential Pressure
Validation Summary
| Conductivity | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes. These filters were then rinsed with 100 L/m2 pure water for 10-20 minutes. The conductivity ranged from 2.79 to 7.51 μS/cm |
| NVR | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes. These filters were then rinsed with 100 L/m2 pure water for 10-20 minutes. The NVR ranged from 667 to 1,314 mg |
| TOC | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes. These filters were then washed with 100 L/m2 pure water for 10-20 minutes. The TOC ranged from 1.12 to 1.55 mg/L |
| pH | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes. These filters were then rinsed with 100 L/m2 pure water for 10-20 minutes. The pH ranged from 6.10 to 6.62 |
| Endotoxin | Several filters were randomly selected from different production batches, then rinsed with 100L/m2 pure water for 10-20minutes, followed by soaking in pure water for 24 hours. The endotoxin level was <0.25 EU/mL as indicated by Tachypleus Amebocyte Lysate test |
| Flow differential pressure | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes. These filters were then rinsed with 100 L/m2 pure water for 10-20 minutes. The experiment was conducted at room temperature. After venting, the flow rate was gradually increased, and the upstream and downstream pressures were observed. Within 8 LPM, the differential pressure was < 0.6 bar |
| Tolerated liquid pressure | Several filters were randomly selected from different production batches and subjected to high-pressure sterilization at 125°C for 60 minutes, twice. The tightness of filter was tested. High-viscosity solution was filtered to increase the upstream and downstream differential pressure of filter. The filter was subjected to forward and reverse pulse pressures at room temperature, followed by rinsing, drying and tightness challenge test. At room temperature, Alidep® Depth Filter can withstand 3.0 bar forward pressure and 2.0 bar reverse pressure |
Ordering Information
Format
- A=Depth filter
Membrane type
- HA01 HC01 HD01 CE01 ACA01
- HA02 HC02 HD02 CE02 ACA02
- …… …… …… …… ……
Filter membrane area
- L23=23 cm²
- P27=0.027 m²
- P54=0.054 m²
- M01=0.11 m²
- M05=0.55 m²
- M11=1.1 m²
Connector type
- L0=Luer connector ( L23 )
- B0=6 mm hose connector ( P27, P54 )
- T1=Flat end connector ( M01, M05, M11 )
Package
- 1=1/PK
- 3=3/PK (L23)
Resource Downloads
Alidep® Depth Filter.pdf
PDF · 641.68 KB · Published on 2026-05-11
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