Synthetics Biology

Synthetics Biology

Alioth's filtration technology enables efficient separation and concentration of synthetic biology products, providing reliable support for fermentation broth clarification and downstream purification.

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Synthetics Biology

Synthetic biology designs and engineers living systems through a "manufacturing by design" approach, serving as a core driver for disrupting traditional manufacturing models and advancing the bio-manufacturing industry. From bio-based materials to innovative drugs, industrial-scale production relies heavily on efficient, scalable separation and purification processes.

From clarification and cell/biomass separation of engineered bacteria/cell culture broth, to the separation, concentration, and purification of target products (such as enzymes and recombinant proteins), Alioth provides a full spectrum of solutions — from depth filtration and pre-filtration to sterile filtration — meeting the diverse needs of synthetic biology manufacturing, ensuring that processes are efficient, stable, and scalable.

Process Map

Plasmid Construction

Plasmid Construction

E. coli Plasmid Amplification

E. coli Plasmid Amplification

Fermentation

Fermentation

Cell Harvest via Hollow Fiber

Cell Harvest via Hollow Fiber

High-Pressure Homogenization

High-Pressure Homogenization

3 kDa MWCO HF Diafiltration Refolding

3 kDa MWCO HF Diafiltration Refolding

PP Filter Clarification

PP Filter Clarification

Inclsion Body Solubilization

Inclsion Body Solubilization

Resuspension & Washing

Resuspension & Washing

Multi-Stage Centrifugation

Multi-Stage Centrifugation

750 kDa UF Aggregate Removal(Optional)

750 kDa UF Aggregate Removal(Optional)

3 kDa MWCO UF Concentration

3 kDa MWCO UF Concentration

Anion / Cation Exchange Chromatography

Anion / Cation Exchange Chromatography

Hydrophobic Interaction Chromatography

Hydrophobic Interaction Chromatography

Reversed-Phase Chromatography

Reversed-Phase Chromatography

Formulation & Final Filling

Formulation & Final Filling

Sterile Filtration

Sterile Filtration

Reduction Filtration

Reduction Filtration

Protectant Addition(Optional)

Protectant Addition(Optional)

Buffer Exchange via 1 kDa DF

Buffer Exchange via 1 kDa DF

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