Peptides
Peptide drugs combine the precision of small-molecule drugs with the activity and specificity of large-molecule biologics, demonstrating enormous potential in metabolic diseases, oncology, and infectious diseases, and representing a major direction in innovative drug R&D.
Alioth's depth filtration and sterile filtration solutions offer outstanding particle retention and microbial removal efficiency, effectively removing insoluble particles, colloids, and microorganisms from peptide solutions — ensuring that peptide drug substances and dosage forms meet the highest standards of purity and safety and comply with stringent pharmacopeial requirements.
Process Map
Purple nodes are clickable and lead to Alioth solutions. Gray nodes are informational only.

Plasmid Construction

CHO Cell Transfection

Fermentation (CHO Cell Culture)
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Disk-stack Centrifugation(Primary Depth Filtration Optional)
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Secondary Depth Filtration
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Anion / Cation Exchange Chromatography

Diafiltration (DF) - Secondary Clarification
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Low-pH Incubation

Affinity Chromatography

Sterile Filtration (0.22 μm)
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Hydrophobic Interaction Chromatography

Virus Removal Filtration (20 nm)

Ultrafiltration / Diafiltration (UF/DF)

Sterile Filtration (0.22 μm)
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Formulation & Final Filling
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